4-Fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) was used as a fluorescence derivatization reagent for amino acids, followed by separation on a core-shell Kinetex C18 column. Lomas continued, “Kinetex Core-Shell Technology delivers huge performance advantages over conventional, fully porous media, such as sharper peaks, reduced run times, and more smiles in the lab. A fast HPLC method using a core-shell particle column was developed for the determination of amino acids. Described in this application note are the details for an investi- gation of basic compound peak shape across four different alkyl. “This new 5-micron phase expands our ever-growing Kinetex product line, which meets customers’ needs from UHPLC to HPLC to small-scale preparative purification,” comments Simon Lomas, brand manager for Phenomenex. Kinetex 5-micron C8 columns are fully scalable to or from the 1.7- and 2.6-micron particles, enabling easy method transferability. In fact, the new 5-micron media provides 90 percent higher average efficiencies compared to the same size fully porous columns with little to no method development. Torrance, CA - Phenomenex Inc., a global leader in the research and manufacture of advanced technologies for the separation sciences, introduces 2.6µm. The largest diameter particle in the family, Kinetex 5-micron core-shell media delivers better performance than 3 and 5-micron fully porous offerings, with no increase in backpressure. The new Kinetex XB-C18 provides a unique selectivity, incorporating two iso-butyl side chains at the base of the C18 ligand to reduce secondary interactions with residual silanols. With the current range of available particle sizes, including 1.3-, 1.7- and 2.6-micron, the same method can be easily transferred from UHPLC to HPLC to Preparative LC with Kinetex Core-Shell Technology.
KINETEX CORE SHELL CODE
Specifically the Kinetex 5-micron C8 meets USP specifications for Classification Code L7, enabling laboratories using 5-micron L7 columns to realize the performance benefits of Kinetex Core-Shell Technology without adjusting their USP methodology. Kinetex core-shell technology meets researchers’ need to improve results and increase productivity without large capital expenditures. Kinetex 5-micron delivers increased resolution and higher efficiencies and extends the scalability and portability of the product line. In combination with Kinetex Core-Shell Technology, this phase gives analysts an excellent high-performance reversed phase alternative for HPLC methods.Īs with the existing Kinetex 5-micron C18 and XB-C18 phases, the new 5-micron C8 core-shell column can be easily substituted into methods that meet United States Pharmacopeia (USP) and European Pharmacopoeia (Ph. Kinetex Core-Shell Technology in the Axia preparative format delivers a performance advantage to customers in pharmaceutical and natural products development. The widely used C8 phase delivers optimum retention for the analysis of hydrophobic and “sticky” compounds. The Kinetex C8 is a core-shell based particle with a bonded silica phase ideal for analysis of very hydrophobic compounds. has introduced the Kinetex C8 5-micron core-shell HPLC column.
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